Fig. 3: Malacidin, a calcium-dependent antibiotic. | Nature Microbiology

Fig. 3: Malacidin, a calcium-dependent antibiotic.

From: Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens

Fig. 3

a, The MIC of malacidin A against MRSA was assessed at various concentrations of calcium and the antibiosis of malacidin A was found to be calcium-dependent. The error bars represent the standard deviation across two replicates over three independent experiments (n = 6). b, Malacidin A is an effective treatment against MRSA in rat cutaneous wound infections. The error bars represent the standard deviation across replicate wounds (n = 4). c, Unlike daptomycin, malacidin A activity against S. pneumoniae is largely unaffected by the presence of pulmonary surfactants. The error bars represent the standard deviation across two replicates over three independent experiments (n = 6). d, After 20 days of repeated exposure to 0.5 × MIC of malacidin A (Mal.), we did not detect any malacidin-resistant S. aureus. Vancomycin (Van.), daptomycin (Dap.) and rifamycin (Rif.) were used as controls in this assay. The error bars represent the standard deviation across three replicates for MIC determination (n = 3).

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