Extended Data Fig. 3: Enhanced inflammation in B6.Sst1S mice requires type I IFN.
From: Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra
a, b, e, f, Protein levels of IL-10 (a), IFNγ (b), TNF (e), and CXCL1 (f) were measured in lungs of Mtb-infected mice at day 25. Combined results of at least three independent infections (a, b, e, f). c, Lung bacterial burden of Mtb-infected mice at day 25 (representative of two independent infections). Input dose: average 100 CFU/mouse. d, CFU corresponding to Fig. 2a and b. Combined results that include those already shown in Figs. 1c, 2c, and Extended Data 3a. Input dose: 10–89 CFU per mouse. g, Neutrophils (CD11b+Ly6G+) from lungs of Mtb-infected mice were enumerated on day 14 and day 25. Combined results of two independent infections. All animals except B6 were bred in-house (a–f); all animals were bred in-house (g). Sample size n (B6, B6.Sst1S, B6.Sst1SIfnar–/–) = 16, 18, 16 (a); 40, 45, 32 (b); 40, 44, 32 (d); 25, 29, 16 (e); 28, 30, 26 (f); 6 for all genotypes (c); 9 for all genotypes (g). Center and error bars show mean and SEM. Analyzed with two-ended Mann–Whitney test (a–g). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.
