Extended Data Fig. 4: IL-1 blockade increases susceptibility in both B6 and B6.Sst1S mice.
From: Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra
a–c, mice were infected with average 15 CFU/mouse and were treated with anti-IL1R1 or isotype control antibodies, and on day 25 the lungs were measured for bacterial burden (a), IL- 1Ra protein levels (b), and IL-1 bioactivity (c). Sample size n (in order shown, from left) = 7, 7, 8, 8 (a); 7, 8, 9, 8 (b-c). d, Mice were infected with average 78 CFU/mouse and IL-1 bioactivity was measured in the lungs at day 25. n = 5 for both samples. e, Mice were infected with average 33 CFU/mouse. IL-1 bioactivity was measured in lung samples collected on the indicated days. n = 6 for all samples except day 36 B6.Sst1S = 4. Day 25 data already shown in Fig. 3d. All animals except B6 were bred in-house (a–c); all were bred in-house (d-e). Center and error bars show mean and SEM. Analyzed with two-ended Mann–Whitney test (a–c). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.
