Extended Data Fig. 5: Homozygous or heterozygous Il1rn deletion protects B6.Sst1S mice from Mtb infection.
From: Type I interferon-driven susceptibility to Mycobacterium tuberculosis is mediated by IL-1Ra
a, Body weights on day 28 of individual mice shown in Fig. 4c. b-c, Mice were infected with Mtb at average 17 CFU/mouse (b) or 60 CFU/mouse (c), and at day 21 lungs were harvested to measure IL-1 bioactivity. d, RT-qPCR on lungs of Mtb-infected mice, sampled at 25 days post-infection. Each graph combined results from 2 independent experiments. e-f, Mice infected with average 20 CFU/mouse were treated with either anti-IL-1R1 antibody or isotype control every 3 days starting 7 days post-infection (e), or anti-IL-1Ra antibody or PBS control every other day starting 3 days post-infection (f). At 25 days post-infection lungs were harvested for measuring bacterial burden. Sample size n (from left as shown) = 11, 12, 22, 10 (a); 6, 8, 11 (b); 6, 8, 10 (c); 11, 14, 11, 5(d); 5, 6, 3, 4, 2, 3 (e); 8, 8, 6, 6 (f). All mice were bred in-house (a, d–f) or all except B6 were bred in-house (b-c); and all except B6 and B6.Sst1S were littermates (a, d, e). Center and error bars show mean and SEM. Analyzed with two-ended Mann–Whitney test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.
