Extended Data Fig. 6: Biotin biosynthesis is a critical fitness determinant for A. baumannii, K. pneumoniae, and P. aeruginosa during a systemic infection with human biotin levels.

Bacterial load in the blood, spleen, kidneys, liver, and lungs following a systemic infection with human biotin levels with (a) A. baumannii (n=8), (b) K. pneumoniae (n=8), (c) P. aeruginosa (n=8), and (d) S. aureus (n=9). Mice were pretreated with streptavidin (2 mg/kg) 1-hour prior to co- infection with a mixed inoculum of wild-type (grey) and BioA deficient (black) bacteria. Box plot whiskers show the minimum to maximum values, the box denotes the interquartile range, and the line in the box shows the median of each group. Groups were analyzed with an unpaired two-tailed t-test and corrected for multiple comparisons with a Holm-Sidak test, * indicates a p<0.01.