Extended Data Fig. 8: Resistance to inhibition of biotin biosynthesis imparts a fitness cost. | Nature Microbiology

Extended Data Fig. 8: Resistance to inhibition of biotin biosynthesis imparts a fitness cost.

From: Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens

Extended Data Fig. 8

Kinetics of (7,8)-di-amino-pelargonic acid production. Initial velocities of (7,8)-di-amino-pelargonic acid production with BioA, BioAW52A, BioAK274A, BioAF393A, and BioAY398A were determined at various (a) (S)-keto-amino-pelargonic acid (KAPA; n=4) and (b) S-adenosyl-L-methionine (SAM; n=6) concentrations. Points show the mean of replicates ± s.d. and the solid lines show a Michaelis-Menten curve. (c) Analysis of the biotin concentrations required to restore growth of BioA deficient A. baumannii passaged into the lowest biotin concentration supporting growth for 14 days. Points show the mean of 8 replicates ± s.d. and the solid lines show a four- parameter dose response curve.

Back to article page