Extended Data Fig. 3: Biotin biosynthesis is dispensable for A. baumannii, K. pneumoniae, P. aeruginosa, S. aureus, and S. Typhimurium during standard infection models. | Nature Microbiology

Extended Data Fig. 3: Biotin biosynthesis is dispensable for A. baumannii, K. pneumoniae, P. aeruginosa, S. aureus, and S. Typhimurium during standard infection models.

From: Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens

Extended Data Fig. 3

Bacterial load in the blood, spleen, kidneys, liver, and lungs following a systemic infection with (a) A. baumannii (AB; n = 5), (b) K. pneumoniae (KP; n=10), (c) P. aeruginosa (PA; n=9), (d) S. aureus (SA; n=6), and (e) S. Typhimurium (ST; n=5). Mice were co-infected with a mixed inoculum of wild- type (grey) and BioA deficient (black) bacteria inoculated by intraperitoneal injection. (f) Bacterial load in the lungs of mice co-infected with a mixed inoculum of wild-type (grey) and ΔbioA (black) bacteria. Infection was established by intranasal administration of 2×107 CFU by micropipette and mice (n=10) euthanized 36 hours later. (g) Bacterial load in the thighs of mice co-infected with a mixed inoculum of wild-type (grey) and BioA deficient (black) A. baumannii (AB; n=6), K. pneumoniae (KP; n=8), P. aeruginosa (PA; n=4), and S. aureus (SA; n=10). 5×105 (KP and SA) or 5×106 (AB and PA) CFU were injected into the thighs of mice. In all instances box plot whiskers show the minimum to maximum values, the box denotes the interquartile range, and the line in the box shows the median.

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