Extended Data Fig. 5: Biotin biosynthesis is a critical fitness determinant for A. baumannii, K. pneumoniae, and P. aeruginosa during a systemic infection with human biotin levels. | Nature Microbiology

Extended Data Fig. 5: Biotin biosynthesis is a critical fitness determinant for A. baumannii, K. pneumoniae, and P. aeruginosa during a systemic infection with human biotin levels.

From: Mimicking the human environment in mice reveals that inhibiting biotin biosynthesis is effective against antibiotic-resistant pathogens

Extended Data Fig. 5

Competitive index (CI) for the co-infection of BioA deficient and wild-type strains of (a) A. baumannii (n=5, 8), (b) K. pneumoniae (n=10, 8), (c) P. aeruginosa (n=9, 8), and (d) S. aureus (n=6, 9) in the blood, spleen, kidneys, liver, and lungs in a standard systemic infection model (light blue) or systemic infection in mice pretreated with streptavidin (2 mg/kg; blue) 1-hour prior to inoculation. CI is calculated by dividing the bacterial load of the query strain (BioA deficient) by the bacterial load of the wild-type strain. Each point represents the CI from an individual mouse and the line indicates the mean. The dotted line represents a CI of 1 indicating the two strains are proliferating equally in vivo. Groups were analyzed with an unpaired two-tailed t-test and corrected for multiple comparisons with a Holm-Sidak test, * indicates a p<0.01.

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