Extended Data Fig. 7: ZIKV-specific T cell functional profiles overtime.
From: Evolution of the innate and adaptive immune response in women with acute Zika virus infection
Detailed representation of the overlapping pie charts presented in Fig. 4d, e. The data represent the different sub-groups of cytokine secreting and cytotoxic CD154+CD4+ (a) and CD69+CD8+ (b) T cells after stimulation with 15-mer overlapping peptide pools covering all ZIKV-proteins, by ex vivo intracellular cytokine stainings (ICSs). Frequencies of IL-2, TNFa, IFNγ and CD107a co-expressing cells are indicated. Baseline signals of IL-2, TNFα, IFNγ and CD107a-expressing cells from unstimulated controls have been subtracted to the stimulated conditions to allow the visualization of ZIKV-specific CD4+ and CD8+ T cell signals. Only time points with detectable CD154+IFNγ+CD4+ (acute n=24, recovery n=26, follow-up n=29) and CD69+IFNγ+CD8+ T cells (acute n=17, recovery n=19, follow-up n=24) from patients CR4965, CR8623, CR8603 and CR8622, as depicted in Fig. 3a, b, have been used for this analysis. Black bars correspond to the median of expression in each condition.
