Extended Data Fig. 4: Humoral immune response to acute ZIKV infection.

Linear regression analysis to model the relationship between plasma anti-ZIKV IgM and IgA detection signals (n=41) using DIACHECK ELISA assays. (b) Plasma detection of anti-ZIKV IgM antibodies using EUROIMMUNE ELISA assay (left chart) and linear regression analysis to model the relationship between plasma anti-ZIKV IgM detection signals obtained with EUROIMMUNE and DIACHECK ELISA assays (n=41) (right chart). (c) Plasma detection of anti-ZIKV IgG antibodies using EUROIMMUNE ELISA assay (left chart) and linear regression analysis to model the relationship between plasma anti-ZIKV IgG detection signals obtained with EUROIMMUNE and DIACHECK ELISA assays (n=41) (right chart). (a-c) Each patient and previous exposure to DENV status, as defined by the positive detection of anti-DENV IgG at symptom onset, is displayed through unique symbols and connecting lines. Gray dashed lines note assay’s detection limits. X-axis, (Time (days)) represents the time from onset of symptoms. Data are expressed as mean values of OD/CO ratios from two independent experiments. Pearson correlation coefficient R and significance p (two-sided) values are reported from the linear regression analysis performed with GraphPad Prism v.6 software.