Extended Data Fig. 6: Growth of 12 Legionella species compared to L. pneumophila in nutrient rich bacteriological medium.
The intracellular growth phenotypes of the 12 Legionella species were not due to differences in growth kinetics as all species grew as well as L. pneumophila in bacteriological medium. L. pneumophila and the indicated species of Legionella were grown in CYE medium and the absorbance at 600 nm was monitored over time. Data are the mean ± standard deviation of 3 biological replicates, each generated from 3 technical replicates. The doubling time (dt) of each species during the exponential growth phase was determined as dt = t/n, where t = time and n = number of generations as describe in Methods. Data are the mean ± standard deviation of 3 biological replicates, each generated from 3 technical replicates; *P < 0.05, two-tailed Student t test relative to L. pneumophila (Source Data for Extended Data Fig. 6).
