Extended Data Fig. 1: L. pneumophila growth in amoebal hosts and macrophages.
a, Intracellular growth cycle of L. pneumophila in A. castellanii, A. polyphaga, H. vermiformis and N. gruberi. Differences in the sets of bacterial genes required for growth in each amoeba were not due to variations in the duration of the intracellular growth cycle, or the cumulative replication, as no correlation between the number of genes identified and the extent of growth was observed and host-specific genes were identified for all hosts examined (Fig. 1). b, The intracellular growth cycle of L. pneumophila in U937 cells is similar to amoebae. In a-b, Host cells were challenged with wild type (WT) or Dot/Icm translocation deficient (dot-) bacteria constitutively expressing the enhanced green fluorescence protein and bacterial growth was monitored by measuring fluorescence over time. Plotted is the relative fluorescence units (RFU) normalized to the WT strain by the RFU at the 1 hour time point (for a, the WT strain in A. castellanii was used). Data are the mean ± standard deviation of 3 biological replicates, each generated from 3 technical replicates (Source Data for Extended Data Fig. 1).
