Extended Data Fig. 4: IDTS mutants exhibit similar phenotypes in primary bone marrow-derived murine macrophages.
a, Intracellular growth of the wild type (WT) and universal IDTS mutant strains in primary bone-marrow-derived macrophages from A/J mice. b, Intracellular replication of auxiliary IDTS mutants. c, Amoebae-specific IDTS genes are a source of redundancy in primary macrophages. In a-c, Macrophages were challenge with the indicated strains and bacterial replication, based on recovered colony forming units (cfus) on solid media from lysed host cells, was monitored 24 hpi and normalized to the WT strain by the number of intracellular bacteria 1 hpi. Data are the mean ± standard deviation of 2–5 biological replicates, each generated from 3 technical replicates; *P < 0.03, 1-way ANOVA, relative to the WT strain (Source Data for Extended Data Fig. 4).
