Extended Data Fig. 6: Effects on inhibition of M. oryzae pathogenicity are proportional to the concentrations of the VLCFA biosynthesis inhibitors, and combination of VLCFAs inhibitors improves efficacy of carbendazim in reducing pathogenicity.
Three VLCFA biosynthesis inhibitors and DMSO control were individually applied in a field trial. Rice leaves were sprayed with Guy11 spore suspension supplemented with an inhibitor at concentrations of 0, 20, 50, 100, 200, 500, 700 and 1000 μΜ, respectively. Blast lesion density was quantitated five-days post infection. a, Metazachlor (n = 27 independent leaves for 0 μΜ, n = 29 for 20 μΜ, n = 29 for 50 μΜ, n = 30 for 100 μΜ, n = 29 for 200 μΜ, n = 29 for 500 μΜ, n = 30 for 700 μΜ, n = 31 for 1000 μΜ; mean ± s.d.). b, Cafenstrole (n = 29 independent leaves for 0 μΜ, n = 29 for 20 μΜ, n = 28 for 50 μΜ, n = 29 for 100 μΜ, n = 29 for 200 μΜ, n = 28 for 500 μΜ, n = 29 for 700 μΜ, n = 29 for 1000 μΜ; mean ± s.d.). c, Diallate (n = 32 independent leaves for 0 μΜ, n = 30 for 20 μΜ, n = 29 for 50 μΜ, n = 30 for 100 μΜ, n = 31 for 200 μΜ, n = 31 for 500 μΜ, n = 31 for 700 μΜ, n = 30 for 1000 μΜ; mean ± s.d.). d, Effects of combination of three VLCFAs inhibitors on pathogenicity of M. oryzae. The three inhibitors were combined in equal amount to a final concentration of 500 μM in Guy11 spore suspension. Then the spore suspension containing combined inhibitors or the three separate inhibitors was inoculated onto rice leaves. DMSO was used as solvent control. Blast lesion density was quantitated after five days of infection (n = 30 independent leaves for DMSO, n = 30 for metazachlor, n = 31 for cafenstrole, n = 30 for diallate, n = 32 for combination of three inhibitors; mean ± s.d.). e, Effects of combination of each VLCFAs inhibitor with carbendazim on pathogenicity of M. oryzae. The three inhibitors were separately combined with carbendazim to produce Guy11 spore suspension which contains 500 μM VLCFAs inhibitor and 8 mM carbendazim. The spore suspension was inoculated onto rice leaves. Carbendazim, metazachlor, cafenstrole and diallate is respectively abbreviated as Car, Met, Caf and Dia. Blast lesion density was quantitated after five-days of infection (n = 31 independent leaves for DMSO, n = 32 for carbendazim, n = 31 for metazachlor, n = 31 for cafenstrole, n = 30 for diallate, n = 29 for combination of carbendazim with metazachlor, n = 31 for combination of carbendazim with cafenstrole, n = 32 for combination of carbendazim with diallate; mean ± s.d.).
