Extended Data Fig. 9: Inhibitors of VLCFAs biosynthesis impaired septin organization and septin-dependent pathogenicity of B. maydis.
a, Microscopic analysis and quantitation of septin puncta detected by BmSep3-GFP at hyphal tips infecting maize leaf at 24 hpi. B. maydis spore suspension was supplemented with 500 μM of each VLCFAs inhibitor and then used for inoculation of maize leaf. DMSO was the solvent control. Percentage of septin dot formation was calculated from 50 hypal tip at 24 hpi (n = 3 independent samples, mean ± s.d., two-sided Student’s t-test). Scale bar, 10 μm. b, Microscopic analysis and quantitation of septin dot detected by BmSep4-GFP from 50 hyphal tips infecting maize leaf at 24 hpi (n = 3 independent samples, mean ± s.d., two-sided Student’s t-test). Scale bar, 10 μm. c, Schematic representation of the targeted deletion of BmSEP3 and BmSEP4 in B. maydis. d, PCR analysis of the targeted deletion in the ΔBmsep3 and ΔBmsep4 mutants. Genomic DNA was analyzed by PCR with primer pairs indicated in panel C. e, Maize leaf infected with B. maydis by a spore suspension spraying assay (n = 7 independent leaves, mean ± s.d., two-sided Student’s t-test).
