Extended Data Fig. 7: Bacterial Cytological Profiling of ΔtolC E.coli treated with known antibiotic compounds on cheese curd agar. | Nature Microbiology

Extended Data Fig. 7: Bacterial Cytological Profiling of ΔtolC E.coli treated with known antibiotic compounds on cheese curd agar.

From: Bacterial–fungal interactions revealed by genome-wide analysis of bacterial mutant fitness

Extended Data Fig. 7: Bacterial Cytological Profiling of ΔtolC E.coli treated with known antibiotic compounds on cheese curd agar.The alternative text for this image may have been generated using AI.

DAPI dye stains DNA and FM4-64 dye stains bacterial membranes. SYTOX green stains nucleic acids but cannot penetrate live cells. Scale bars represent 2 µm. Testing of each antibiotic at four concentrations was performed once, and cells from the edges of zones of clearing were imaged for at least 5 fields from each condition to ensure consistency in phenotype.

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