Extended Data Fig. 8: Cladogram of translated dinoflagellate tubulin contigs with reference MMETSP dinoflagellate proteins (left) shown alongside tubulin gene expression in the mesopelagic compared to euphotic zone (log2 average fold change) (middle) and average expression levels (TPM-normalized transcript abundance) in the mesopelagic (right).

Alpha-tubulin is shown in blue, Gamma-tubulin in green, and beta-tubulin in orange. Reference sequences were aligned using MUSCLE v3.8 and the maximum-likelihood tree was created using RAxML v8.2.11 with the PROTGAMMALG model and 100 bootstrap replicates. Contigs were placed on the reference tree using pplacer v1.1alpha19. The cladogram is visualized with the R package ggtree v1.16.0.