Extended Data Fig. 3: ICP0 interacts SLFN5 via the carboxy terminal region of SLFN5.
a, Subcellular localization of SLFN5 mutants was analyzed. HEK293T cells were transfected with plasmids encoding GFP-SLFN5 (full-length or various deletion mutants). At 24 hours after transfection, the cells monitored for the GFP signals. Scale bar, 50 µm. b, HEK293T cells were co-transfected with plasmids encoding various version of GFP-SLFN5 and ICP0 ΔRING or cytoplasmic ICP0 mutant (cICP0, also called D8) ΔRING, as indicated. The cells were subject to co-IP with anti-GFP Ab, followed by immunoblotting. Immunoblot and immunofluorescence images show representative data from n = 3 biologically independent experiments. c, SLFN5 interaction with ICP0 maps to a part of the intrinsicly disordered region of SLFN5. The SLFN5 and SLFN11 protein sequences were analyzed for disorder tendency using IUPred2A. The domain structure of SLFN is shown above. The green bar indicates an interaction region of ICP0 or a homolgous region of SLFN11 with SLFN5. Red lines indicate unique disorder region of SLFN5.
