Extended Data Fig. 8: FttA-mediated transcription termination completes the archaeal transcription cycle.
From: FttA is a CPSF73 homologue that terminates transcription in Archaea
Promoter-directed assembly of pre-initiation complexes requires RNAP, TFB and TBP and is often assisted by TFE. De novo RNA synthesis permits promoter escape and transcription initiation factors are replaced by transcription elongation factors TFS and Spt4-Spt5. The absence of a nuclear compartment permits translation initiation and the normal coupling of the archaeal transcription and translation apparatuses throughout transcription of the gene or operon, but this coupling is disrupted by translation termination. The exposed nascent transcript likely permits loading of FttA to TECs and FttA activity mediates cleavage of nascent transcripts and release of RNAP to solution.
