Extended Data Fig. 2: FttA is highly conserved and shares structural and sequence homology with eukaryotic CPSF73.
From: FttA is a CPSF73 homologue that terminates transcription in Archaea
Recombinant FttA (the protein product of T. kodakarensis TK1428; TK1428p) is 73.5 Kda, 85˚C thermotolerant and free of contaminating proteins. Lane M contains molecular weight standards in Kda. Data shown are from a single experiment. b, The crystal structure of FttA from Pyrococcus horikoshii (PDB: 3AF5)17 shown in chainbow coloring (N-terminus in purple to C-terminus in red) reveals two N-terminal KH-domains attached by a linker to a C-terminal MBL fold. Alpha-carbons of highly conserved residues in archaeal FttA homologues are shown in colored spheres. c, The MBL-fold of FttA is nearly structural identical to the MBL-fold of the human CPSF73 protein (PDB: 2I7T)16.
