Extended Data Fig. 2: Growth patterns in different media compositions.
From: Genetically engineered control of phenotypic structure in microbial colonies
a, Kymographs indicating inter-image differences (IIDs, see Methods) as in Fig. 1c during the establishment of a steady state growth pattern in 170μm deep traps for growth media containing different amounts of glucose (the standard concentration is 0.2% w/v). For lower concentrations, growth extends further into the back of the trap with a smoother transition between regions of growth and no growth. Kymographs on the right of the black line show consistent behavior in the smaller (140μm deep) traps (the dashed line indicates the end of the trap). b, Distance from the mouth of the microfluidic trap at which the inter-image difference (IID) shown in a drops below detectable levels. For this purpose, the IID profile was averaged over 4 h of steady-state growth. The threshold for growth detection was chosen as the lowest possible level that safely detected the no-growth region in standard growth medium.
