Extended Data Fig. 8: Trypanosomes are unable to colonise the PV and produce lower MG infections in previously blood fed flies.
From: Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus
a, Representative microscopy images of proventriculi from teneral and refractory tsetse (i.e. blood fed three times before receiving a trypanosome-infected bloodmeal) at three dpi in bright field, a maximum intensity projection of three confocal z-stacks of eGFP-expressing T. brucei strain J-10 (green) and merge. Scale bar 200 μm. b, Infection prevalence rates (percentage) of proventriculi (PV) and midguts (MG) from teneral (green) and refractory (purple) flies at three dpi and five dpi. Sample size is shown as numbers above bars, one experiment.
