Extended Data Fig. 4: UHRF1 depletion induces latency III and induces ICAM-1 in EBV+ but not EBV- B-cells.
From: DNA methylation enzymes and PRC1 restrict B-cell Epstein–Barr virus oncoprotein expression
a, Volcano plot visualization of -Log10 (p-value) statistical significance (y-axis) and Log2 (fold-change in mRNA abundance) in Rael cells that expressed control or UHRF1 sgRNAs. Data are from n = 2 of biologically independent RNA-seq datasets. P value and log fold change were generated with DESeq under default settings with Wald test and Normal shrinkage, respectively. Values for selected genes upregulated (red) or downregulated (blue) by UHRF1 sgRNA are highlighted. b, Enrichr pathway analysis of gene sets significantly upregulated in MUTU III versus MUTU I (gray bars), or by UHRF1 versus control sgRNA expression in MUTU I (black bars) or Rael (blue bars). Shown are the adjusted p-values from Enrich analysis of triplicate RNAseq datasets using Fisher exact test. See also Source Data for Extended Data Fig. 4. c, Heatmap representation of abundances of mRNAs encoding latency III antigens in Rael BL with control or UHRF1 sgRNAs. Shown are data from n = 2 biologically independent replicates. d, FACS analysis of total cell ICAM-1 and LMP1 expression in Rael cells with control (left) or UHRF1 sgRNA #2 (right). e, Immunoblot analysis of WCL from EBV- (left) or EBV+ Akata BLs with the indicated sgRNA expression. f, FACS analysis of plasma membrane ICAM-1 expression in the indicated cell lines with control or UHRF1 sgRNA #2 expression. KEM I and Rael are EBV+ BL. REH is an EBV-negative B-cell acute lymphoblastic leukemia cell line. Shown below are immunoblots of WCL from KEM I BLs with the indicated sgRNA expression. All FACS plots and blots are representative of n = 3 biologically independent replicates.
