Extended Data Fig. 5: Single particle cryo-EM analysis of R2M2S1 bound to Ocr. | Nature Microbiology

Extended Data Fig. 5: Single particle cryo-EM analysis of R2M2S1 bound to Ocr.

From: Structural insights into assembly, operation and inhibition of a type I restriction–modification system

Extended Data Fig. 5

a,b, Representative cryo-EM micrographs of R2M2S1-Ocr (a) and R2M2S1-Ocr-Grafix (b). Experiments were performed two times independently with similar results. c,d, Representative reference-free 2D-class averages of R2M2S1-Ocr (c) and R2M2S1-Ocr-Grafix (d). Experiments were performed two times independently with similar results. e, Cryo-EM density maps of R1M2S1-OcrRAS-2-local colored on the basis of the local resolution. f,g, The gold standard Fourier shell correlation (FSC) curves of R2M2S1-Ocr (f) and R2M2S1-Ocr-Grafix (g). h, Angular distribution of particles included in the final 3D reconstruction of R1M2S1-OcrRAS-2-local. i,j, Data-processing workflows for R2M2S1-Ocr (i) and R2M2S1-Ocr-Grafix (j).

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