Extended Data Fig. 6: Single particle cryo-EM analysis of R2M2S1 bound to ArdA. | Nature Microbiology

Extended Data Fig. 6: Single particle cryo-EM analysis of R2M2S1 bound to ArdA.

From: Structural insights into assembly, operation and inhibition of a type I restriction–modification system

Extended Data Fig. 6

a,b, Representative cryo-EM micrographs of R2M2S1-ArdA (a) and R2M2S1-ArdA-Grafix (b). Experiments were performed two times independently with similar results. c,d, Representative reference-free 2D-class averages of R2M2S1-ArdA (c) and R2M2S1-ArdA-Grafix (d); Experiments were performed two times independently with similar results. e,f, Cryo-EM density maps of R1M2S1-ArdARAS (e) and R2M2S1-ArdATS-4 (f) colored on the basis of the local resolution. g, The gold standard Fourier shell correlation (FSC) curves of R2M2S1-ArdA complexes (upper panel); Corrected Fourier shell correlation (FSC) of R2M2S1-ArdATS-4 (blue solid line), FSC of the unmasked map (red dashed line), FSC of the masked map (green dashed line) and FSC of the randomized masked map (orange dashed line) (lower panel). h,i, Angular distribution of particles included in the final 3D reconstruction of R1M2S1-ArdARAS (h) and R2M2S1-ArdATS-4 (i). j,k, Data-processing workflows for R2M2S1-ArdA (j) and R2M2S1-ArdA-Grafix (k).

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