Extended Data Fig. 1: Expression of the Nup62 dimerization construct in cells does not alter normal cell physiology. | Nature Microbiology

Extended Data Fig. 1: Expression of the Nup62 dimerization construct in cells does not alter normal cell physiology.

From: Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus

Extended Data Fig. 1

a, HeLa cells were transduced simultaneously with lentiviral vectors driving expression of Nup62DG or mCherry-estrogen receptor alpha (ERα). Imaging fields were selected that contain cells expressing only ERα (marked by asterisk) or both ERα and Nup62DG. Live cell imaging was performed after addition of estradiol (E2) to mediate nuclear translocation of ERα. Images acquired every four minutes for a total of one hour. b, Similar experiment as in (a) except the cells were treated with the homodimerizing drug (HD) to block NPC transport along with E2 treatment. c, Accumulation of mCherry-ERα fluorescence in the nuclear region was monitored in Nup62DG+ and Nup62DG- cells treated with E2 using ImageJ plugin Time Series Analyzer V3. Depicted mean values from three independent experiments (±SEM). d, similar quantification as in (c) in cells treated with E2 and HD. Error bar represents SEM. Statistical significance was assessed using Two-way ANOVA and Bonferroni post test. P<0.05 was considered significant in our experiments.

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