Extended Data Fig. 3: HIV-1 infection induces Nup62 relocalization and colocalization with HIV-1 cores in the cytoplasm. | Nature Microbiology

Extended Data Fig. 3: HIV-1 infection induces Nup62 relocalization and colocalization with HIV-1 cores in the cytoplasm.

From: Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus

Extended Data Fig. 3

a, Nup62 localization in uninfected HeLa cells. b, HeLa cells synchronously infected with VSVg- R7ΔEnvmCherry at 3 h post infection, fixed and stained for Nup62 (green), HIV-1 capsid protein p24 (red), and DAPI (blue) for cell nuclei. Enlarged view of colocalization (boxed region) between Nup62 and HIV-1 capsid protein p24 shown in the bottom panel and indicated by arrows. c, Quantification of cytoplasmic Nup62, as described in methods section and Supplementary Fig. 2a. 20 or more cells analyzed per sample. Data averaged from three independent experiments. Error bar represents SEM. d, Quantification of the percent p24 colocalizing with Nup62 in HeLa cells. 20 or more cells analyzed per sample. Data averaged from three independent experiments. Error bar represents SEM. Statistical significance was assessed using Two-way ANOVA and Bonferroni post test. P<0.05 was considered significant in our experiments.

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