Extended Data Fig. 5: Monitoring reverse transcription with a low dose of RT inhibitor nevirapine to mirror the level of inhibition induced by Nup62DG blockade.
THP1 cells expressing the Nup62DG were differentiated to macrophages and infected with VSVg- R7ΔEnvmCherry and NIK monitored by HD addition (red) as described in Fig. 2. To monitor replication kinetics, cells incubated with HIV-1 reverse transcriptase inhibitor Nevirapine (NVP, 1.6 µM) at different time’s post infection as depicted (green) and washed off at 24 h. Infection measured as described in Fig. 2. Bar graphs depict data from a single experiment and line graphs depict normalized and average data (±SEM) as described in Fig. 2 from three independent experiments. NT represents no treatment.
