Extended Data Fig. 7: Effect of GHP-88309 treatment on HPIV3 and MeV primary transcription.
From: Orally efficacious broad-spectrum allosteric inhibitor of paramyxovirus polymerase
Effect of GHP-88309 treatment on HPIV3 and MeV primary transcription. a-c, GHP-88309 significantly reduced levels of MeV P (a), H (b), and L (c) mRNA transcripts at 12 hours after infection. d, GHP-88309 significantly reduced the synthesis of HPIV3 Le RNA 2 hours after infection. e, f, GHP-88309 did not significantly alter the HPIV3 (e; 2 hours after infection) and MeV (f; 4 hours after infection) primary mRNA transcription gradients. Values represent relative changes compared to vehicle treated samples (a-d) or relative changes compared to HPIV3 N (e) or MeV P (f) mRNA levels. Experiments were conducted in at least three biological repeats, determined in duplicate each. In (a–f), symbols represent individual biological repeats, columns denote means, and error bars represent SD. Statistical significance was determined by one-way ANOVA (a–d) or two-way ANOVA (e, f) using Dunnett’s multiple comparison post-hoc test (two-sided).
