Extended Data Fig. 8: GHP-88309 inhibits de novo initiation of RNA synthesis at the promoter.
From: Orally efficacious broad-spectrum allosteric inhibitor of paramyxovirus polymerase
GHP-88309 inhibits de novo initiation of RNA synthesis at the promoter. a, b, RSV-derived RNA template (a) that was used in Fig. 2j and is capable of back-priming depicted in (b). c, Repeat result of the MeV RdRP assay with the RSV-derived template shown in Fig. 2; originals are provided in Source Data file (Plemper_SourceData_Fig2_Originals_Repeats.pdf). d, MeV-derived RNA template, incapable of back-priming. e, MeV RdRP assay with the MeV-derived template shown (d). The experiment was performed three times with similar results. Originals and repeats are provided in (Plemper_SourceData_ED_Fig8_Originals_Repeats.pdf). Higher molecular weight material in (e) likely represents poly-adenylation consistent with previous results obtained with a similar template and NiV polymerase complexes48.
