Extended Data Fig. 6: Tunicamycin and ascorbic acid treatments affect appressorial penetration and adhesion.
a, Penetration rates at 30 h.p.i. for appressoria formed on rice leaf sheath surfaces from germinating WT spores treated or untreated (NT) with Tunicamycin (5 µg/mL) at the indicated time points. Values are the mean penetration rates determined for n=50 appressoria, repeated in triplicate. Bars are standard deviation. Significant differences of the means at a given time are denoted by different lowercase letters (one-way ANOVA, F4,10 = 173.00, p < 0.0001). b, Penetration rates at 30 h.p.i. for appressoria formed on rice leaf sheath surfaces from spores treated with 0.25 µM ascorbic acid. NT, no treatment. Values are the mean penetration rates determined for n=50 appressoria, repeated in triplicate. Bars are standard deviation. Significant differences of the means comparing WT and Δsps1 at a given treatment are denoted by different lowercase letters (two-way ANOVA, F1, 8 = 74.30. NT, p < 0.0001; + Ascorbic acid p < 0.0001). Significant differences of the means within WT or within Δsps1 are denoted by different uppercase letters (letters (two-way ANOVA, F1, 8 = 74.30. WT, p = 0.9450; Δsps1, p < 0.0001). c, Rates of WT appressorial adhesion in 96-well plates at 24 h.p.i. after germinating spores were treated at 6 h.p.i. with 2.5 μM ascorbic acid (asc) and/ or 5 μg/ mL Tunicamycin (Tun). This was repeated in triplicate for each treatment. Significant differences of the means are denoted by different lowercase letters (one-way ANOVA, F2,6 = 80.35, p < 0.0001). Error bars indicate standard deviation.
