Extended Data Fig. 1: Δsps1 physiology compared to WT.
a, Sporulation rates of WT and the Δsps1 mutant strains were not significantly different (unpaired two-tailed t-test, t = 0.1346 df = 4, p = 0.8994) following 14 days of growth on CM. Value are the mean of spores liberated from three independent plates. Bars are standard deviation. b, WT and Δsps1 strains after ten days of growth on 88 mm petri-dishes containing complete media (CM) or 1 % (w/v) glucose minimal media (GMM), as indicated. Three independent plate test experiments gave similar results. c, Appressorium formation rates on detached rice leaf sheath surfaces. Values are means obtained by determining how many of n=50 germinating conidia had formed appressoria by 24 h.p.i., repeated in triplicate. Bars are standard deviation. Bars with different letters are significantly different (unpaired two-tailed t-test, t = 24.04, df = 4, p < 0.0001). d, Appressorial penetration rates on detached rice leaf sheaths. Values are means obtained by determining how many of n=50 appressoria had penetrated rice cuticles by 30 h.p.i., as determined by observing primary hyphae or early IH in underlying rice cells, repeated in triplicate. Bars are standard deviation. Bars with different letters are significantly different (unpaired two-tailed t-test, t = 94.75 df = 4, p < 0.0001). c, d, Leaf sheaths were inoculated with 1 x 105 spores per mL of the indicated strain.
