Extended Data Fig. 5: Loss of spermine induces the unfolded protein response (UPR) and cell wall integrity (CWI) response.

ER stress induces the UPR and CWI. Relative transcript abundances of UPR-associated genes (LHS1, KAR2, SCJ1, SIL1, PDI1)²⁹ and a cell wall integrity-associated gene (CCW12)³⁰ were upregulated in the Δsps1 strain grown in liquid complete media without spermine supplementation compared to WT. Levels of the indicated genes were reduced in Δsps1 mycelia when grown in the presence of 1 mM spermine. Expression levels were normalized against M. oryzae β-tubulin gene expression. Quantitative real-time PCR assays were carried out in triplicate. Template controls did not show amplification. Error bars indicate standard deviation. Significant differences of the means comparing WT and Δsps1 at the indicated treatment are denoted by different lowercase letters (two-way ANOVA, LHS1 F_{1, 8} = 432.9. NT, p < 0.0001. + Spermine, p = 0.0327; KAR2 F_{1, 8} = 22.10. NT, p < 0.0001. + Spermine, p = 0.0096; SIL1 F_{1, 8} = 42.12. NT, p < 0.0001. + Spermine, p = 0.2416; PDI1 F_{1, 8} = 45.40. NT, p < 0.0001. + Spermine, p = 0.2443; SCJ1 F_{1, 8} = 26.42. NT, p < 0.0001. + Spermine, p = 0.7101; CCW12 F_{1, 8} = 49.28. NT, p < 0.0001. + Spermine, p = 0.0151). Significant differences of the means within WT or within Δsps1 are denoted by different uppercase letters (two-way ANOVA, LHS1 F_{1, 8} = 432.9. WT, p = 0.9781. Δsps1, p < 0.0001; KAR2 F_{1, 8} = 22.10. WT, p = 0.0055. Δsps1, p < 0.0001; SIL2 F_{1, 8} = 42.12. WT, p = 0.0328. Δsps1, p < 0.0001; PDI1 F_{1, 8} = 45.40. WT, p = 0.0129. Δsps1, p < 0.0001; SCJ1 F_{1, 8} = 26.42. WT, p = 0.7503. Δsps1, p < 0.0001; CCW12 F_{1, 8} = 49.28. WT, p = 0.9996. Δsps1, p < 0.0001).

Source data