Extended Data Fig. 5: Candida species induce type I interferon signalling independently of apoptosis. | Nature Microbiology

Extended Data Fig. 5: Candida species induce type I interferon signalling independently of apoptosis.

From: Candida pathogens induce protective mitochondria-associated type I interferon signalling and a damage-driven response in vaginal epithelial cells

Extended Data Fig. 5: Candida species induce type I interferon signalling independently of apoptosis.

The proportion of healthy, necrotic, and apoptotic vaginal epithelial cells (ECs) 3 and 24 hours post-infection (hpi) with Candida in (a) A431 vaginal ECs used throughout this study and (b) primary vaginal ECs. Treatment with 1.2 µM staurosporine was used as a positive control. c, Mitochondrial membrane potential change of primary vaginal ECs at 1 hpi, positive control CCCP 100 μM. d, Relative expression (RT-qPCR) of selected Interferon-Stimulated Genes (ISGs) in C. albicans-infected ECs where apoptosis was induced with 1.2 µM staurosporine at 3 hpi. All values are presented as mean ± SD of n = 3 independent experiments. Statistical significance is indicated as: *, p ≤ 0.05; ***, p ≤ 0.001; ***, p ≤ 0.0001 (one-way ANOVA with Dunnett’s multiple comparisons test (c-d).; ‘calb’ denotes C. albicans, ‘cglab’ - C. glabrata, ‘cpar’ - C. parapsilosis, ‘ctrop’ - C. tropicalis.

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