Extended Data Fig. 4: Complementation of ΔftsZ1 ΔftsZ2.

a, Phase-contrast images (left) and Coulter cytometry (right) of strains based on H. volcanii ID112 (ΔftsZ1 ΔftsZ2), plus pTA962-based plasmids expressing the indicated ftsZ genes, sampled during mid-log growth with the indicated concentrations of Trp. The same dataset for the wild-type control (H26 + pTA962) is shown in all graphs as a reference. Scale bars, 5 μm. b, Corresponding western blot analyses of FtsZ1 and FtsZ2 protein levels in total cell extracts of the indicated strains. The data shown is representative of two independent experiments.