Extended Data Fig. 8: FtsZ1-mCh localization in ftsZ2-mutant strains.
a, Demonstration of the automated image analysis procedure for determining FtsZ localization parameters. Cell outlines were obtained (red), and the fluorescence (FtsZ1-mCherry in yellow) was quantified by averaging the intensity on the transverse axis to create a longitudinal intensity profile. Gaussian peaks were fitted to the detected localizations and a spline fit to the background. The localization thickness (W) was taken as the width of the fitted Gaussian peaks at half height (μm), and the intensity (I) was taken as the integrated peak area (per μm across the cell). See Methods for further details. b, Histograms for cells with the indicated number of localizations versus cell length for ΔftsZ2 + FtsZ1-mCh. Colored lines indicate the lengths of cells that have the indicated relative number of localizations per unit length. c, Violin plots of the thickness of FtsZ1-mCh localization in the indicated strain backgrounds; the median is indicated by a white dot, the thick bar is the interquartile range, and thin bar is the 9th-91st percentile range. The data shown are representative of at least two independent experiments. Explanation of the experiment using the ΔftsZ2 + FtsZ2.D231A-GFP + FtsZ1-mCh strain is given in the Supplementary results and discussion and Extended Data Fig. 10.
