Extended Data Fig. 10: Co-localization studies of wild-type FtsZ and T7-loop mutants.
Fluorescence microscopy (overlay of GFP and mCherry channels; co-localization appears white) (left) and cell size/shape analysis plots (right) for the indicated strains (grown with 0.2 mM Trp) containing one tagged wild-type protein and the alternate tagged the T7-loop mutant. The data shown are representative of at least two independent experiments. In panels (b) and (d), results with cells grown with 1 mM Trp is shown in the lower insets; the morphology percentages for these were: (b) 95.4% wild-type-like, 3.2% giant plates, 0.9% filaments, and 0.5% debris (n = 439), and (d) 40.7% wild-type-like, 25.5% giant plates, 29.1% filaments, and 5.1% debris (n = 196). Scale bars, 5 μm.
