Fig. 3: Virulence contribution of the Stp complex and plant protein interactors.

a, Seven-day-old maize seedlings were infected with the indicated strains. Disease symptoms were scored 12 d.p.i. Strain SG200stp1-YFP_C stp3-YFP_N expresses indicated BiFC fusion proteins from genes tagged in their native locus. All other columns display SG200 with increasing numbers of ectopic insertions of stp1-YFP_N and stp3-YFP_C in addition to the native untagged copies. All data represent the mean of n = 3 biologically independent experiments. Total numbers of infected plants are shown above the respective columns. b, Plant interactors of the Stp complex shown by a volcano plot illustrating the P values versus log₂ protein abundance ratios between Stp1–HA and mCherry–HA for co-IP/MS experiments, which were performed in biological triplicates with material isolated 3 d.p.i. and extracted with high detergent mix of maize infected with FB1stp1–HA × FB2stp1–HA. Displayed are all proteins for which at least four peptides were detected. Coloured dots show the indicated proteins. Significance was calculated using Student’s t-test. The blue- and grey-shaded areas indicate P values ≤0.01 and ≤0.05, respectively, and an x-axis value (log₂-intensity difference of mCherry versus Stp1) below −3.