Extended Data Fig. 6: DINOR functions as a lncRNA in C. auris morphogenesis.
From: LncRNA DINOR is a virulence factor and global regulator of stress responses in Candida auris
a, Deleting DINOR led to filamentation. Images show the cellular and colony morphologies of dinorΔ and dinorΔ:DINOR cells. WT and T004840:LEU2 cells were included as controls. Scale bar, 10 μm. Columns on the right side show the percentages of yeast and filamentous cells of the indicated strains grown in liquid or solid YPD medium (n = 200). Error bars, s.d. from the mean of three independent experiments. b, Short ORFs (1-5) within DINOR identified using ORF Finder (http://www.ncbi.nlm.nih.gov/orffinder/). c, Expression of each putative ORF under the control of PENO1 failed to restore the yeast morphology of dinorΔ cells. Images show the cellular and colony morphologies of dinorΔ:PENO1-ORF1, dinorΔ:PENO1-ORF2, dinorΔ:PENO1-ORF3, dinorΔ:PENO1-ORF4, and dinorΔ:PENO1-ORF5 cells. WT and dinorΔ cells were included as controls. Scale bar, 10 μm. Columns at the bottom show the percentages of yeast and filamentous cells of the indicated strains grown in liquid or solid YPD medium (n = 200). Error bars, s.d. from the mean of three independent experiments. d, Mutating the start codon ATG of each ORF individually to AAG did not affect DINOR’s function. DINOR carrying the ATG-AAG mutation was integrated into the genome of dinorΔ. Images show the cellular and colony morphologies of dinorΔ:DINORT1115A, dinorΔ:DINORT1021A, dinorΔ:DINORT815A, dinorΔ:DINORT528A, and dinorΔ:DINORT319A cells. WT, dinorΔ, and dinorΔ:DINOR cells were included as controls. Scale bar, 10 μm. White vertical lines mark single nucleotide mutations. Columns at the bottom show the percentages of yeast and filamentous cells of the indicated strains grown in liquid or solid YPD medium (n = 200). Error bars, s.d. from the mean of three independent experiments.
