Extended Data Fig. 1: PB transposition in C. auris.
From: LncRNA DINOR is a virulence factor and global regulator of stress responses in Candida auris
a, The PB system. White vertical lines indicate hyperactive mutations. The PB transposon cassette PB[LEU2] is integrated within ARG4. Arrows indicate the PCR primers used to detect PB excision. b, PB[LEU2] is stable at the ARG4 locus in CauW08 under non-inducing conditions. Gel electrophoresis of PCR products amplified from the genomic DNA of 24 colonies obtained from three independent experiments using primers shown in a. Wild-type (WT) cells were included as a control. c, Transposition efficiency. Error bars, s.d. from the mean of three independent experiments. d, Density curves of PB-specific site counts per 10 kb in the three libraries. e, Correlations of PB site counts per 10 kb between the three libraries.
