Extended Data Fig. 5: Identification of DINOR in C. auris.
From: LncRNA DINOR is a virulence factor and global regulator of stress responses in Candida auris
a, qPCR analysis of 004840 and 004841 expression in WT and T004840:LEU2 cells. Overnight cultures of WT and T004840:LEU2 strains were collected for RNA isolation. The expression levels were normalized against GAPDH, and that of the WT sample was set as 1. Error bars represent s.d. from the mean of three independent experiments. b, Effect of 004840 and 004841 on the filamentous phenotype of T004840:LEU2 cells. (Right top) Description of genotypes of 004840:LEU2, 004841:LEU2, and 004840:SAT1 004841:LEU2 cells. Arrows indicate the primers used for genotyping. Bottom panel, Cellular and colony morphologies of 004840:LEU2, 004841:LEU2, and 004840:SAT1 004841:LEU2 cells. WT and T004840:LEU2 cells were included as controls. Scale bar, 10 μm. Right panel, Gel electrophoresis results of PCR-based genotyping of the indicated strains. WT cells were included as a control. The experiment was independently repeated three times with similar results. c, qPCR analysis of four non-overlapping regions downstream of 004840. qPCR templates derived from total RNA of WT cells with or without reverse transcription. ACT1 and GAPDH were included as controls. d, Detection of the potential transcript downstream of 004840 by primer-walking PCR. Top, Schematic description of primers (arrows) used for primer-walking PCR. Bottom, PCR templates were prepared from the total RNA of WT cells with or without the reverse transcription step. Genomic DNA was included as a control. The experiment was independently repeated three times with similar results.
