Extended Data Fig. 7: PTI-associated phosphorylation sites of RBOHD are involved in resistance to opportunistic pathogens.
From: The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves
a, ROS production of A. thaliana Col-0, rbohD, rbohF, rbohD/RBOHD, rbohD/RBOHD-S39AS339AS343A, rbohD/RBOHD-S343A-S347A, rbohD/RBOHD-S343A-S347A, rbohDrbohF/RBOHD, rbohDrbohF/RBOHD-S343A-S347A and rbohDrbohF, after treatment with 100 nM flg22. ROS production was measured in leaf discs from soil-grown plants with a luminol-based assay and expressed as integrated luminescence over 45 min (AU, arbitrary units). Box plots show the median with upper and lower quartiles and whiskers present 1.5x interquartile range. b, Fresh weight of Col-0, rbohD, rbohD/RBOHD, and rbohD/RBOHD-S343A-S347A inoculated with individual strains of Xanthomonas spp. Leaf131, Leaf148, Pst hrcC-, and Pst wild-type or mock-inoculated with 10 mM MgCl2. Germ-free 10-day-old seedlings were inoculated with OD = 0.02 of the respective strains. Box plots show the median with upper and lower quartiles and whiskers present 1.5x interquartile range. Significant differences were calculated between the mutants and their respective controls (n = 20; two-tailed Mann-Whitney U-test). Brackets above bar plots indicate comparison groups with p-values displayed above and fold-change below. Data from two independent experiments are shown in separate graphs.
