Extended Data Fig. 6: Indifference of rpoB H526Y cells to steady-state ppGpp concentration underlies its fitness cost.
a, Sensitivity of the indicated strains to A22, 5FUd and high temperature. b, Robust growth of wild type and rpoB H526Y versus auxotrophy of relAspoT on minimal agar plates. c, Proteomic analysis of differentially expressed proteins between rpoB H526Y and relAspoT (ppGpp0). Venn diagram presenting the overlap between differentially expressed proteins in rpoB H526Y and relAspoT cells in comparison to the wild type strain. A full list of genes is given in Supplementary Table 6. The total number of proteins detected across all three strains is 2174. The overlap between differentially regulated proteins in rpoB H526Y and relAspoT cells, in comparison to wild type cells, has p-values of 1.1×10−4 and 1.29×10−8, respectively, for up- and down-regulated proteins. d, ppGpp concentration in lysates prepared from two biological replicates of wild type and rpoB H526Y.
