Extended Data Fig. 9: Mean RNAP pause frequency in the upp genetic background.
NET-seq analysis of RNAP pausing in wild type, upp, and uppH526Y double mutant. For each gene, the mean number of pauses per kilobase gene (the pause frequency) was calculated. The midline in each box denotes the aggregated median frequency for the top 3500 expressed genes for each condition. The lower and upper ends of the box denote Q1 and Q3, respectively. The whiskers span 1.5*(Q3–Q1) from each side of the box. **** p-value <0.0001, N.S not significant. p-values are based on the student t-test (two-sided, equal variance). For each condition, n = 2 biologically independent replicates of NET-seq experiments.
