Extended Data Fig. 1: Genome-wide fitness cost analysis in RifR rpoB H526Y.
a, Genome-wide normalized transposon insertion density (IPKM) values. Each data point corresponds to a single gene. Genes are ordered on the x-axis based on distance from oriC, and are stratified according to previous annotation of gene essentiality and dispensability in wild type E. coli MG1655. b, Insertion saturation analysis. Each bar provides the number of genes with a given transposon insertion saturation value. Gene essentiality is colour-coded. Total number of reads and overall saturation percentage are indicated. c, Distribution of gene number as a function of IPKM values. Per gene, the IPKM value is the sum of reads mapped to transposon insertion sites per mean number of TA sites in kilobases per million reads. Gene essentiality is colour-coded. d, positive correlation between fold IPKM value of top cost-mitigating genes included in Supplementary Table 1 and fold IPKM values of the same genes from Supplementary Table 2. e, Gene Ontology (GO) enrichment analysis for biological processes that are either cost mitigating (blue) or compounding (red). f, Gene browser comparative view of transposon insertion sites of cost mitigating genes in wild type (black) and rpoB H526Y (blue) at the zapB, dacA, and relA loci. g, same as in (f) for cost-compounding genes in wild type (black) and RifR H526Y (red) at the rsgA, rlmE, and gpp loci. h, Growth in LB of single gene knockouts in genetic background of wild type or H526Y.
