Extended Data Fig. 3: Specific synthetic lethal interaction between RifR rpoB H526Y and yebA zapA (ΔΔ). | Nature Microbiology

Extended Data Fig. 3: Specific synthetic lethal interaction between RifR rpoB H526Y and yebA zapA (ΔΔ).

From: Analysing the fitness cost of antibiotic resistance to identify targets for combination antimicrobials

Extended Data Fig. 3

a, Transduction of RifR rpoB H526Y to yebA zapA depends on a complementation plasmid expressing zapA. b, Poor co-transduction efficiency of RifR rpoB H526Y cells to multiple double knockout combinations of top cost mitigating genes from the Tn-seq analysis. Donor P1 phage lysates were prepared either from RifR rpoB H526Y (with linked kanamycin resistance cassette downstream of the 3’ of rpoC) or from RifS strain with the same linked selectable marker. The efficiency of transducing the Km cassette to indicated recipients (wild type and selected double knockouts) was calculated by dividing the number of transductants to the number of recipient cells. c, serial dilution and plating of the indiciated strains. Unlike the synthetic lethal interaction between RifR rpoB H526Y with yebA zapA (ΔΔ), the RifR rpoB S531F is compatible with the yebAzapA double deletion without any growth defect.

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