Fig. 5: SDSI + AmpSeq is used to identify sample swaps and contamination. | Nature Microbiology

Fig. 5: SDSI + AmpSeq is used to identify sample swaps and contamination.

From: Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows

Fig. 5: SDSI + AmpSeq is used to identify sample swaps and contamination.

a, Intentional SDSI contamination experiment (run in duplicate) assessing whether different ratios of contamination between SDSI 87 and SDSI 94 (SDSI 87:SDSI 94) were detectable with the SDSI + AmpSeq method. b, Examples of experimental errors that were caught using the SDSI + AmpSeq method. c, Top: distance matrix showing pairwise differences between the 17 complete genomes assembled from this sample set. Putative cluster samples are in bold. Bottom: spike-in counts for each of the 24 samples and water controls in this sequencing batch.

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