Extended Data Fig. 1: Multi-parametric flow cytometry of T cells from individuals who received the BNT162b12 vaccine or overcame COVID-19.

Peripheral blood CD4 T cell responses to SARS-CoV2 spike peptide pool, SEB, and control conditions (unstimulated) of representative responders and the five low/non-responders to two-dose BNT162b2 vaccination, and of reconvalescent COVID-19 patients were analyzed by flow cytometry at the indicated time points. All plots show data of gated CD4 T cells also stained for CD40L and IFNγ as markers of activated T cells. The frequency of CD40L/IFNγ co-expressing cells among total CD4 T cells is indicated in each plot.