Extended Data Fig. 7: Auxotrophs export more non-essential metabolites and generate a rich metabolic environment conducive for drug tolerance.
(a) Percentage change in expression of proteins involved in different amino acid biosynthetic pathways in the sorted histidine, leucine, uracil and methionine auxotrophs. Amino acid pathway annotations were taken as per the metabolic model, iMM904. (b) FBA analysis assessing amino acid exchange in auxotrophic versus protrophic subpopulations. The FBA analysis indicates that with the increased export of metabolites from auxotrophs, the degree of metabolite exchange within communal cells increases. Pink indicates export of metabolites from auxotrophs, blue indicates export of metabolites from prototrophs. (c) Qualitative analysis of concordance between fluxes (FBA analysis) and protein expression (proteomics analysis) in the auxotrophic versus prototrophic subpopulations.
