Extended Data Fig. 3.: Knocking down HKT increases 3-HK level.

a, Relative expression levels of HKT in non-injected and dsRNA injected mosquitoes. The expression level of HKT was normalized to S7. The relative expression levels of HKT in dsHKT and dsGFP mosquitoes were normalized to the gene’s expression in non-injection group, respectively. Data are presented as mean ± s.e.m (n = 8). Results from one of two independent experiments are shown. b, Relative amount of 3-HK in dsGFP and dsHKT mosquitoes right before blood meal. Data are presented as mean ± s.e.m (n = 10). Significance was determined using ANOVA with Tukey’s test in (a) and two-sided Student’s t-test in (b).